Evaluation of the Apoptotic and Scolicidal Effects of Crude and Flavonoid Extracts of Allium noeanum on Protoscolices and Hydatid Cyst Wall

Background: Hydatidosis is a disease, caused by the larval phase of Echinococcus granulosus. It considered serious condition, as cyst rupture can release protoscolices into host body, leading to formation secondary cysts. Surgery main approach for treatment this disease. Some natural compounds, which are safe options with few side effects, have been assessed their scolicidal activities. Objectives: Allium noeanum native herb Markazi Province, Iran, has used traditional certain diseases. However, its anti-parasitic property not investigated so far. The present study aimed evaluate plant evaluating and apoptotic effects two types herbal extract (crude flavonoid) on hydatid Methods: cysts were obtained from abattoirs, drained under sterile conditions. suspension walls immunohistochemical evaluations. In addition, A. was collected Shazand Mountains. crude flavonoid extracts prepared maceration chromatography methods, respectively. Immunohistochemistry conducted detection caspase-3 activity using commercial kit. Data analyzed in Excel SPSS, statistical significance defined P < 0.05. Results: 100% concentration (0.49 gr/mL) death protoscolices. On other hand, all concentrations led parasites. According analyses, each type showed different dose- time-dependent rates (P 0.001). lower exposed flavonoids, compared those extract. Conclusions: study, against confirmed. also found that effect more than